Circular Dichronism Spectroscopy
- To determine the specific rotation of a sugar using a polarimeter
- To understand the effect of chiral substances on plane polarized light as a function of wavelength
- To study the Optical Rotatory Dispersion (ORD) of some chiral substances
- To study the Circular Dichroism of some substances with chiral chromophores
- To Study the operational details of a CD spectrometer
- To Study the effect of Secondary Structure elements on CD spectrum of proteins
- To deconvolute the CD spectrum of a given protein solution and to classify it in terms of its secondary structure elements
- To study the thermal stability of proteins
- To study the effect of chemical denaturants on protein stability
- To study the comparison between protein unfolding and protein aggregation in the presence of chemical denaturing agents